Study 4 Downloads

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Performed by the CPTAC Experimental Design and Statistics: Verification Studies Working Group assessed the utility of multiple reaction monitoring (MRM) coupled with stable-isotope dilution mass spectrometry (SID-MS) for quantification of proteins in human plasma with a particular focus on the reproducibility and transferability of protein-based MRM assays across multiple laboratories. Towards this goal, CPTAC Study 4 was implemented to assess intra- and inter-laboratory performance of an MRM/SID-MS assay designed to quantitate 10 target peptides representing 7 target proteins that were spiked into human plasma. This initial (unpublished) “feasibility” study developed standard operating procedures (SOPs) for sample preparation, data acquisition and analysis. A follow-up study that systematically evaluated the effect of sources of variability within the study design, including spiking of the 7 target proteins into human plasma and digestion at each individual site is described in Study 7.

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• Study 8: Discovery WG
  (Yeast, No SOP) 
• Study 7: Verification WG (published study)
  
• Study 6: Discovery WG
  (Yeast+Sigma UPS 1, SOP 2.2) 
• Study 5: Discovery WG
  (Yeast+BSA, SOP 2.1) 
• Study 4: Verification WG (feasibility study)
  
• Study 3: Discovery WG
  (Yeast, SOP 2.0) 
• Study 2: Discovery WG
  (NCI-20, SOP 1.0) 
• Study 1: Discovery WG
  (NCI-20, No SOP)